Adsul, M. G. and Varma, A. J. and Gokhale, D. V. (2007) Lactic acid production from waste sugarcane bagasse derived cellulose. Green Chemistry, 9. pp. 58-62.

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Production of L(+)lactic acid from sugarcane bagasse cellulose, one of the abundant biomass materials available in India, was studied. The bagasse was chemically treated to obtain a purified bagasse cellulose sample, which is much more amenable to cellulase enzyme attack than bagasse itself. This sample, at high concentration (10%), was hydrolyzed by cellulase enzyme preparations (10 FPU g21 cellulose) derived from mutants generated in our own laboratory. We obtained maximum hydrolysis (72%), yielding glucose and cellobiose as the main end products. Lactic acid was produced from this bagasse cellulose sample by simultaneous saccharification and fermentation (SSF) in a media containing a cellulase enzyme preparation derived from Penicillium janthinellum mutant EU1 and cellobiose utilizing Lactobacillus delbrueckii mutant Uc-3. A maximum lactic acid concentration of 67 g l21 was produced from a concentration of 80 g l21 of bagasse cellulose, the highest productivity and yield being 0.93 g l21 h21 and 0.83 g g21, respectively. The mutant Uc-3 was found to utilize high concentrations of cellobiose (50 g l21) and convert it into lactic acid in a homo-fermentative way. Considering that bagasse is a waste material available in abundance, we propose to valorize this biomass to produce cellulose and then sugars, which can be fermented to products such as ethanol and lactic acid.

Item Type: Article
Subjects: Biological Sciences
Depositing User: Dr D.V. Gokhale
Date Deposited: 06 Feb 2016 09:32
Last Modified: 06 Feb 2016 09:32

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