Pradhan, Ketaki and Gadgil, Mugdha (2012) Effect of addition of ‘carrier’ DNA during transient protein expression in suspension CHO culture. Cytotechnology, 64. pp. 613-622.

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Transient protein expression using polyethyleneimine as a transfection agent is useful for the rapid production of small amounts of recombinant proteins. It is known that an increase in extracellular DNA concentration during transfection can lead to a nonlinear increase in intracellular DNA concentration. We present an approach that utilizes this nonlinearity to decrease the amount of plasmid required for productive transfections. Through addition of non coding ‘carrier’ DNA to increase total DNA concentration during transfection, we report a statistically significant increase in protein (IgG) expression per unit plasmid used for transfection. This approach could be useful to increase protein yields for large scale transfections under conditions where plasmid availability is limited.

Item Type: Article
Subjects: Chemical Engineering Sciences
Depositing User: Dr Mugdha Gadgil
Date Deposited: 15 Jul 2015 09:29
Last Modified: 15 Jul 2015 09:29

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