Gupta, Reetika and Prabhune, Asmita (2012) Structural determination and chemical esterification of the sophorolipids produced by Candida bombicola grown on glucose and α- linolenic acid. Biotechnology Letters, 34 (4). pp. 701-707. ISSN 0141-5492

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Abstract

The extracellular surface-active glycolipids produced by the yeast, Candida bombicola when grown on glucose and α-linolenic acid were analyzed using high performance liquid chromatography (HPLC) with electro-spray ionization (ESI) and collision induced dissociation (CID) mass spectrometry. The analysis confirmed that the sophorolipid (SL) mixture contained three different forms of C18:3 SL molecules. These three different forms were free acid, lactone and diacetylated lactone, which had not been reported previously. Also a minor amount of diacetylated lactone form of C18:1 SL molecule was detected. Further, the SL mixture was subjected to chemical esterification reaction with sodium methoxide. The reaction product was analyzed with ESI-MS and confirmed to be the single homogenous esterified product containing C18:3 moieties in its fatty acid chain.

Item Type: Article
Subjects: Biological Sciences
Divisions: UNSPECIFIED
Depositing User: Ms Reetika Gupta
Date Deposited: 26 Jun 2012 04:25
Last Modified: 11 Dec 2015 07:21
URI: http://ncl.csircentral.net/id/eprint/1031

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